Cell Assay Protocol 004: MTT assay to Test the Effect of RNA Nanoparticles within Cells
Design your experiment with the control groups, concentrations and technical repeats. Seed the cells in 96-well plate at about 40% confluency, and culture at 37°C in humidified air containing 5% CO2 overnight.
Incubate cells with RNA nanoparticles in complete cell culture medium for 48-72 hours. Suspend the RNA nanoparticles in fresh cell culture medium with a 10% fetal bovine serum (FBS) at the indicated concentrations and added to the cells for incubation at 37°C for 48-72 hours.
Add 15 ?L of dye solution to each well and incubate at 37°C for 4 h; add 100 ?L of solubilization/stop solution to each well and incubated at room temperature for 2 h for color development.
Record the absorbance at 570 nm using a microplate reader (Synergy 4; BioTek Instruments, Inc.). Calculate the cell viability relative to the absorbance of cells in only control (viability of cells in only control = 100%).
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